Antioxidant Activity of Six Essential Oils and its Molecules in Ecuadorian Andean Medicinal Plants

Paco Fernando Noriega Rivera, Edison E. Díaz, Ivana Marcela Villegas Paucarina, Karla Natalia Pozo Morales, Priscila Pamela Guerrero Toapanta, Pablo Andres Guerra Cajas, Christian Fabricio Larenas Uria

Research output: Contribution to journalArticle


Background: This research values the antioxidant activity and its responsible molecules in six essential oils from medicinal plants in the Ecuadorian Andes. Methods: The chemical composition of essential oils was determined using gas chromatography coupled mass spectrometry. For evaluated the antioxidant activity of essential oils was use tree spectrophotometric methods: diphenyl-1-picrylhydrazyl (DPPH), 2,2’-Azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and β-Carotene bleaching test. The essential oils with good activity were determined the responsible molecules using the Bioautographic HP-TLC-DPPH method. Results: The scavenging capacity of the radicals was assessed with DPPH and ABTS methods, the best results were found in the oils of M. mollis IC50 DPPH 2.80 mg/ml and IC50 ABTS 0.205 mg/mL and in A. glutinosa IC50 DPPH 12.972 mg/mL and IC50 ABTS 0.321 mg/mL, the results were compared with a pattern of natural reference in this case, the essential oil of T. vulgaris IC50 DPPH 0.474 mg/mL and IC50 ABTS 0.272 mg/mL. The evaluation of the antioxidant activity was determined by the β-carotene bleaching test, the most notable activity results were from M. mollis IC50 0.119 mg/mL, A. glutinosa IC50 0.062 mg/mL and B. latifolia IC50 0,064 mg/mL. DPPH bioautography revealed the active molecules antioxidants in oils for M. mollis were thymol acetate (7.73%) and carvacrol acetate (24.52%), for A. glutinosa was γ-muurolene (2.68%), and for B. latifolia Z-caryophyllene (2.99%), aristolochene (0.11%) and cis-cadin-4-en-7-ol (4.11%). Conclusion: The results of antioxidant activity shown in descending order that the essential oils of: M. mollis, A. glutinosa and B. latifolia, are those with the highest activity using the DPPH and ABTS methods. The β-Carotene bleaching test method confirms the 3 oils as the most active in the following order: A. glutinosa, B. latifolia and M. mollis. An antioxidant bioautographic study identified the molecules responsible for the activity in three essential oils with good activity.
Translated title of the contributionActividad antioxidante de seis aceites esenciales y sus moléculas en plantas medicinales andinas ecuatorianas
Original languageEnglish (US)
Pages (from-to)1-9
Number of pages9
JournalTMR Modern Herbal Medicine
Issue number6
StatePublished - 28 Jul 2023


  • Andean essential oils
  • Minthostachys mollis
  • Bioautography antioxidant
  • Antioxidant activity

CACES Knowledge Areas

  • 215A Biochemistry


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