TY - JOUR
T1 - Optimization of an Alternative Culture Medium for Phycocyanin Production from Arthrospira platensis under Laboratory Conditions
AU - Freire Balseca, Daniel Alberto
AU - Castro Reyes, Kimberly Susana
AU - Maldonado Rodríguez, María Elena
N1 - Publisher Copyright:
© 2024 by the authors.
PY - 2024/2/10
Y1 - 2024/2/10
N2 - Arthrospira platensis, known as spirulina, is a cyanobacterium with multiple nutritional benefits, as it contains substantial amounts of proteins, fatty acids, and pigments. However, the production of this microalga has faced significant challenges, primarily related to the cost and composition of the required culture medium for its optimal growth. This study focused on optimizing two nitrogen sources (urea and potassium nitrate) to maximize the growth of A. platensis and the production of phycocyanin, a photosynthetic pigment of significant commercial value. Optimization was performed using the response surface methodology (RSM) with a central composite design (CCD). Analysis of variance (ANOVA) was employed to validate the model, which revealed that the different concentrations of urea were statistically significant (p < 0.05) for biomass and phycocyanin production. However, potassium nitrate (KNO3) showed no significant influence (p > 0.05) on the response variables. The RSM analysis indicated that the optimal concentrations of KNO3 and urea to maximize the response variables were 3.5 g L−1 and 0.098 g L−1, respectively. This study offers valuable perspectives for the efficient production of A. platensis while reducing production costs for its cultivation on a larger scale.
AB - Arthrospira platensis, known as spirulina, is a cyanobacterium with multiple nutritional benefits, as it contains substantial amounts of proteins, fatty acids, and pigments. However, the production of this microalga has faced significant challenges, primarily related to the cost and composition of the required culture medium for its optimal growth. This study focused on optimizing two nitrogen sources (urea and potassium nitrate) to maximize the growth of A. platensis and the production of phycocyanin, a photosynthetic pigment of significant commercial value. Optimization was performed using the response surface methodology (RSM) with a central composite design (CCD). Analysis of variance (ANOVA) was employed to validate the model, which revealed that the different concentrations of urea were statistically significant (p < 0.05) for biomass and phycocyanin production. However, potassium nitrate (KNO3) showed no significant influence (p > 0.05) on the response variables. The RSM analysis indicated that the optimal concentrations of KNO3 and urea to maximize the response variables were 3.5 g L−1 and 0.098 g L−1, respectively. This study offers valuable perspectives for the efficient production of A. platensis while reducing production costs for its cultivation on a larger scale.
KW - central composite design
KW - phycocyanin
KW - pigment
KW - potassium nitrate
KW - response surface methodology
KW - spirulina
KW - urea
UR - http://www.scopus.com/inward/record.url?scp=85187273857&partnerID=8YFLogxK
U2 - 10.3390/microorganisms12020363
DO - 10.3390/microorganisms12020363
M3 - Article
C2 - 38399769
AN - SCOPUS:85187273857
SN - 2076-2607
VL - 12
JO - Microorganisms
JF - Microorganisms
IS - 2
M1 - 363
ER -