In-vivo ANTI-INFLAMMATORY ACTIVITY OF A TOPICAL FORMULATION WITH ACTIVE PRINCIPLES ON ESSENTIAL OILS OF Cannabis sativa L. (CANNABIS) AND Baccharis latifolia (RUIZ & PAV) PER. (CHILCA)

Paco Noriega; Tatiana Idrobo; Mishell Pintag; Diego Vinueza; Christian Larenas

doi:10.17163/lgr.n37.2023.02

In-vivo ANTI-INFLAMMATORY ACTIVITY OF A TOPICAL FORMULATION WITH ACTIVE PRINCIPLES ON ESSENTIAL OILS OF Cannabis sativa L. (CANNABIS) AND Baccharis latifolia (RUIZ & PAV) PER. (CHILCA)

Paco Noriega, Tatiana Idrobo, Mishell Pintag, Diego Vinueza, Christian Larenas

Research and Development Group in Sciences Applied to Biological Resources (GIDCARB)

Research output: Contribution to journal › Article › peer-review

Abstract

Essential oils of Cannabis sativa (cannabis) and Baccharis latifolia (chilca), were used as anti-inflammatory ingredients in an ointment for topical application. Different formulations designed based on these two essential oils were evaluated in-vivo to measure efficacy, using the feet swelling induction method in rats, and an over-the-counter formulation with 1% diclofenac active ingredient was used as a positive control. The chemical evaluation of the two oils yielded the following main components, for chilca oil: liguloxide 14.02%, andro encecalinol 9.84%, kesane 7.53%, limonene 5.6% and Z-cadin-4-en-7-ol with 5.03%; while for cannabis essential oil: E-caryophillene 27.91%, myrcene 21.19%, a-pinene 8.05%, a-humulene 8.03%, limonene 7.18%, terpinolene 7.12% and b-pinene 4.68%. The results of the research indicate that those formulas that combined two essential oils in the formulation at 1%, are the ones with the highest anti-inflammatory activity. Statistically, the significance is high in relation to the positive control in those whose oil composition is essential oil of cannabis 75% and chilca oil 25%; and essential oil of cannabis 50% and chilca oil 50%. The other formulas have activity, but this is similar to the commercial formula used as control. Based on the results, it is possible to propose both natural products as anti-inflammatories, and to foresee the design and commercialization of topical pharmaceutical drugs using these two essential oils.

Translated title of the contribution	ACTIVIDAD ANTIINFLAMATORIA in-vivo DE UNA FORMULACIÓN TÓPICA CON PRINCIPIOS ACTIVOS DE ACEITES ESENCIALES DE Cannabis sativa L. (CÁÑAMO) Y Baccharis latifolia (RUIZ & PAV) PER. (CHILCA)
Original language	English
Pages (from-to)	23-33
Number of pages	11
Journal	Granja
Volume	37
Issue number	1
DOIs	https://doi.org/10.17163/lgr.n37.2023.02 https://doi.org/10.17163/lgr.n37.2023.02
State	Published - 1 Mar 2023

Bibliographical note

Funding Information:
The authors thank Universidad Politécnica Salesiana by the funding awarded to Grupo de Investigación y Desarrollo en Ciencias Aplicadas a los Recursos Biológicos (GIDCARB), by the project entitled: Optimización de procesos extractivos de metabolitos secundarios de Cannabis sativa (cáñamo) fase II (Optimization of extractive processes of secondary metabolites of Cannabis sativa (cannabis) phase II).

Funding Information:
The authors thank Universidad Politécnica Salesia-na by the funding awarded to Grupo de Investiga-ción y Desarrollo en Ciencias Aplicadas a los Recur-sos Biológicos (GIDCARB), by the project entitled: Optimización de procesos extractivos de metaboli-tos secundarios de Cannabis sativa (cáñamo) fase II (Optimization of extractive processes of secondary metabolites of Cannabis sativa (cannabis) phase II).

Publisher Copyright:
©2023, Universidad Politécnica Salesiana, Ecuador.

Keywords

anti-inflammatory activity
anti-inflammatory synergy
Baccharis latifolia
Cannabis sativa
essential oils

CACES Knowledge Areas

8515A Biodiversity

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title = "In-vivo ANTI-INFLAMMATORY ACTIVITY OF A TOPICAL FORMULATION WITH ACTIVE PRINCIPLES ON ESSENTIAL OILS OF Cannabis sativa L. (CANNABIS) AND Baccharis latifolia (RUIZ & PAV) PER. (CHILCA)",

abstract = "Essential oils of Cannabis sativa (cannabis) and Baccharis latifolia (chilca), were used as anti-inflammatory ingredients in an ointment for topical application. Different formulations designed based on these two essential oils were evaluated in-vivo to measure efficacy, using the feet swelling induction method in rats, and an over-the-counter formulation with 1% diclofenac active ingredient was used as a positive control. The chemical evaluation of the two oils yielded the following main components, for chilca oil: liguloxide 14.02%, andro encecalinol 9.84%, kesane 7.53%, limonene 5.6% and Z-cadin-4-en-7-ol with 5.03%; while for cannabis essential oil: E-caryophillene 27.91%, myrcene 21.19%, a-pinene 8.05%, a-humulene 8.03%, limonene 7.18%, terpinolene 7.12% and b-pinene 4.68%. The results of the research indicate that those formulas that combined two essential oils in the formulation at 1%, are the ones with the highest anti-inflammatory activity. Statistically, the significance is high in relation to the positive control in those whose oil composition is essential oil of cannabis 75% and chilca oil 25%; and essential oil of cannabis 50% and chilca oil 50%. The other formulas have activity, but this is similar to the commercial formula used as control. Based on the results, it is possible to propose both natural products as anti-inflammatories, and to foresee the design and commercialization of topical pharmaceutical drugs using these two essential oils.",

keywords = "anti-inflammatory activity, anti-inflammatory synergy, Baccharis latifolia, Cannabis sativa, essential oils",

author = "Paco Noriega and Tatiana Idrobo and Mishell Pintag and Diego Vinueza and Christian Larenas",

note = "Publisher Copyright: {\textcopyright}2023, Universidad Polit{\'e}cnica Salesiana, Ecuador.",

year = "2023",

month = mar,

day = "1",

doi = "10.17163/lgr.n37.2023.02",

language = "English",

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T1 - In-vivo ANTI-INFLAMMATORY ACTIVITY OF A TOPICAL FORMULATION WITH ACTIVE PRINCIPLES ON ESSENTIAL OILS OF Cannabis sativa L. (CANNABIS) AND Baccharis latifolia (RUIZ & PAV) PER. (CHILCA)

AU - Noriega, Paco

AU - Idrobo, Tatiana

AU - Pintag, Mishell

AU - Vinueza, Diego

AU - Larenas, Christian

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Y1 - 2023/3/1

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AB - Essential oils of Cannabis sativa (cannabis) and Baccharis latifolia (chilca), were used as anti-inflammatory ingredients in an ointment for topical application. Different formulations designed based on these two essential oils were evaluated in-vivo to measure efficacy, using the feet swelling induction method in rats, and an over-the-counter formulation with 1% diclofenac active ingredient was used as a positive control. The chemical evaluation of the two oils yielded the following main components, for chilca oil: liguloxide 14.02%, andro encecalinol 9.84%, kesane 7.53%, limonene 5.6% and Z-cadin-4-en-7-ol with 5.03%; while for cannabis essential oil: E-caryophillene 27.91%, myrcene 21.19%, a-pinene 8.05%, a-humulene 8.03%, limonene 7.18%, terpinolene 7.12% and b-pinene 4.68%. The results of the research indicate that those formulas that combined two essential oils in the formulation at 1%, are the ones with the highest anti-inflammatory activity. Statistically, the significance is high in relation to the positive control in those whose oil composition is essential oil of cannabis 75% and chilca oil 25%; and essential oil of cannabis 50% and chilca oil 50%. The other formulas have activity, but this is similar to the commercial formula used as control. Based on the results, it is possible to propose both natural products as anti-inflammatories, and to foresee the design and commercialization of topical pharmaceutical drugs using these two essential oils.

KW - anti-inflammatory activity

KW - anti-inflammatory synergy

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KW - Cannabis sativa

KW - essential oils

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DO - 10.17163/lgr.n37.2023.02

M3 - Article

AN - SCOPUS:85165976377

SN - 1390-3799

VL - 37

SP - 23

EP - 33

JO - Granja

JF - Granja

IS - 1

ER -

In-vivo ANTI-INFLAMMATORY ACTIVITY OF A TOPICAL FORMULATION WITH ACTIVE PRINCIPLES ON ESSENTIAL OILS OF Cannabis sativa L. (CANNABIS) AND Baccharis latifolia (RUIZ & PAV) PER. (CHILCA)

Abstract

Bibliographical note

Keywords

CACES Knowledge Areas

Access to Document

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