Exploiting the capability of preparing nanostructured bifunctional terminated SrTiO3 substrates (SrO and TiO2), the surface properties have been locally tuned by employing a double bottom-up strategy which combines the use of chemically nanopatterned substrates with molecular self-assembly. The dynamics of surface diffusion that allows SrO and TiO2 chemical-termination nano-patterning of the SrTiO3 is first addressed. Second, termination-dependent heterogeneous nucleation is used to demonstrate that stearic acid selectively grows on the TiO2 terminated terraces. This adsorption improves the frictional properties and modifies the surface contact potential. The possibility of simultaneously tailoring at the nanoscale different surface properties of widely employed oxide substrates is promising for building up new devices useful for emerging applications. © the Owner Societies.