The Effect of Ascorbic and Citric Acids in the in Vitro Establishment of Solanum betaceum to Prevent Phenolic Oxidation

Katherine Sofía Salazar-Vega; Sebastián Andrés Pillalaza Montalvo; Ivonne Vaca-Suquillo

doi:10.1007/978-981-16-4126-8_19

The Effect of Ascorbic and Citric Acids in the in Vitro Establishment of Solanum betaceum to Prevent Phenolic Oxidation

Katherine Sofía Salazar-Vega, Sebastián Andrés Pillalaza Montalvo, Ivonne Vaca-Suquillo

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

Tamarillo (Solanum betaceum) is an Andean woody plant. Due to the chemical composition and high-nutritional value of its fruits, it is considered a viable option for research and mass production through plant tissue culture. This species is susceptible to phenolic oxidation caused by mechanical damage such as cuts made to obtain axillary buds as explants. In order to solve this problem, explants were cut and added into a solution of citric acid and ascorbic acid at a concentration of 100 ppm, respectively; subsequently, the disinfection protocol was conducted to avoid contamination in the phase of in vitro establishment. This research aims to analyze the response of axillary buds and seeds. Phenolic oxidation, plantlet regeneration, number and coloration of leaves, as well as callus induction, were evaluated. Axillary buds showed 8% of phenolic oxidation, while seeds did not present any. The highest plantlet regeneration (68.42%), leaf coloration (3/5) and number of leaves (3.26) were obtained by axillary buds. The phenolic oxidation showed by buds (8%) was very low compared to previous literature.

Original language	English
Title of host publication	Communication, Smart Technologies and Innovation for Society - Proceedings of CITIS 2021
Editors	Álvaro Rocha, Paulo Carlos López-López, Juan Pablo Salgado-Guerrero
Publisher	Springer Science and Business Media Deutschland GmbH
Pages	199-205
Number of pages	7
ISBN (Print)	9789811641251
DOIs	https://doi.org/10.1007/978-981-16-4126-8_19
State	Published - 2022
Event	7th International Conference on Science, Technology and Innovation for Society, CITIS 2021 - Virtual, Online Duration: 26 May 2021 → 28 May 2021

Publication series

Name	Smart Innovation, Systems and Technologies
Volume	252
ISSN (Print)	2190-3018
ISSN (Electronic)	2190-3026

Conference

Conference	7th International Conference on Science, Technology and Innovation for Society, CITIS 2021
City	Virtual, Online
Period	26/05/21 → 28/05/21

Bibliographical note

Funding Information:
Acknowledgements The authors would like to thank the Universidad Politécnica Salesiana for supporting this research.

Publisher Copyright:
© 2022, The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd.

Keywords

Antioxidant
Axillary bud
Phenolic oxidation

Access to Document

10.1007/978-981-16-4126-8_19

Cite this

Salazar-Vega, K. S., Pillalaza Montalvo, S. A., & Vaca-Suquillo, I. (2022). The Effect of Ascorbic and Citric Acids in the in Vitro Establishment of Solanum betaceum to Prevent Phenolic Oxidation. In Á. Rocha, P. C. López-López, & J. P. Salgado-Guerrero (Eds.), Communication, Smart Technologies and Innovation for Society - Proceedings of CITIS 2021 (pp. 199-205). (Smart Innovation, Systems and Technologies; Vol. 252). Springer Science and Business Media Deutschland GmbH. https://doi.org/10.1007/978-981-16-4126-8_19

Salazar-Vega, Katherine Sofía ; Pillalaza Montalvo, Sebastián Andrés ; Vaca-Suquillo, Ivonne. / The Effect of Ascorbic and Citric Acids in the in Vitro Establishment of Solanum betaceum to Prevent Phenolic Oxidation. Communication, Smart Technologies and Innovation for Society - Proceedings of CITIS 2021. editor / Álvaro Rocha ; Paulo Carlos López-López ; Juan Pablo Salgado-Guerrero. Springer Science and Business Media Deutschland GmbH, 2022. pp. 199-205 (Smart Innovation, Systems and Technologies).

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abstract = "Tamarillo (Solanum betaceum) is an Andean woody plant. Due to the chemical composition and high-nutritional value of its fruits, it is considered a viable option for research and mass production through plant tissue culture. This species is susceptible to phenolic oxidation caused by mechanical damage such as cuts made to obtain axillary buds as explants. In order to solve this problem, explants were cut and added into a solution of citric acid and ascorbic acid at a concentration of 100 ppm, respectively; subsequently, the disinfection protocol was conducted to avoid contamination in the phase of in vitro establishment. This research aims to analyze the response of axillary buds and seeds. Phenolic oxidation, plantlet regeneration, number and coloration of leaves, as well as callus induction, were evaluated. Axillary buds showed 8% of phenolic oxidation, while seeds did not present any. The highest plantlet regeneration (68.42%), leaf coloration (3/5) and number of leaves (3.26) were obtained by axillary buds. The phenolic oxidation showed by buds (8%) was very low compared to previous literature.",

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Salazar-Vega, KS, Pillalaza Montalvo, SA & Vaca-Suquillo, I 2022, The Effect of Ascorbic and Citric Acids in the in Vitro Establishment of Solanum betaceum to Prevent Phenolic Oxidation. in Á Rocha, PC López-López & JP Salgado-Guerrero (eds), Communication, Smart Technologies and Innovation for Society - Proceedings of CITIS 2021. Smart Innovation, Systems and Technologies, vol. 252, Springer Science and Business Media Deutschland GmbH, pp. 199-205, 7th International Conference on Science, Technology and Innovation for Society, CITIS 2021, Virtual, Online, 26/05/21. https://doi.org/10.1007/978-981-16-4126-8_19

The Effect of Ascorbic and Citric Acids in the in Vitro Establishment of Solanum betaceum to Prevent Phenolic Oxidation. / Salazar-Vega, Katherine Sofía; Pillalaza Montalvo, Sebastián Andrés; Vaca-Suquillo, Ivonne.
Communication, Smart Technologies and Innovation for Society - Proceedings of CITIS 2021. ed. / Álvaro Rocha; Paulo Carlos López-López; Juan Pablo Salgado-Guerrero. Springer Science and Business Media Deutschland GmbH, 2022. p. 199-205 (Smart Innovation, Systems and Technologies; Vol. 252).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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AU - Vaca-Suquillo, Ivonne

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Salazar-Vega KS, Pillalaza Montalvo SA, Vaca-Suquillo I. The Effect of Ascorbic and Citric Acids in the in Vitro Establishment of Solanum betaceum to Prevent Phenolic Oxidation. In Rocha Á, López-López PC, Salgado-Guerrero JP, editors, Communication, Smart Technologies and Innovation for Society - Proceedings of CITIS 2021. Springer Science and Business Media Deutschland GmbH. 2022. p. 199-205. (Smart Innovation, Systems and Technologies). doi: 10.1007/978-981-16-4126-8_19

The Effect of Ascorbic and Citric Acids in the in Vitro Establishment of Solanum betaceum to Prevent Phenolic Oxidation

Abstract

Publication series

Conference

Bibliographical note

Keywords

Access to Document

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