TY - JOUR
T1 - Quantification of FEND and ITUDI Anti-fungal Lipopeptide Gene Expression in Bacillus megaterium using RT-qPCR
AU - Chiluisa-Utreras, Viviana Pamela
AU - Jara, Katherine Alejandra Medrano
AU - Paredes, Andrés Sebastián Cadena
AU - Acurio, Ramiro Daniel
N1 - Publisher Copyright:
© 2020 Journal of Pure and Applied Microbiology. All rights reserved.
PY - 2020/12
Y1 - 2020/12
N2 - Phytopathogenic diseases are a major concern in modern agriculture, and for decades, pesticides have been used to prevent potential damage. Bacillus megaterium is proposed as a biological controlling agent, and gene expression of the lipopeptide genes FEND and ITUDI was assessed using RT-qPCR. Inhibition effects of B. megaterium on Alternaria sp. and Botrytis sp. were examined over a period of nine days, which confirmed the potential use of this bacterium to counteract these two pathogens. In addition, expression of FEND and ITUDI genes was assessed over nine days in the aforementioned dual cultures and inhibition tests. FEND expression in B. megaterium increased 20.16-fold in response to Alternaria sp., and ITUDI expression increased 3.20-fold in response to Botrytis sp. on day five of incubation. These results were corroborated by gene expression data obtained from B. megaterium during fermentation, where FEND and ITUDI gene expression increased 95.14- and 18.70-fold, respectively. In conclusion, B. megaterium can increase lipopeptide synthesis when exposed to these particular phytopathogens and can significantly increase the respective expression during fermentation.
AB - Phytopathogenic diseases are a major concern in modern agriculture, and for decades, pesticides have been used to prevent potential damage. Bacillus megaterium is proposed as a biological controlling agent, and gene expression of the lipopeptide genes FEND and ITUDI was assessed using RT-qPCR. Inhibition effects of B. megaterium on Alternaria sp. and Botrytis sp. were examined over a period of nine days, which confirmed the potential use of this bacterium to counteract these two pathogens. In addition, expression of FEND and ITUDI genes was assessed over nine days in the aforementioned dual cultures and inhibition tests. FEND expression in B. megaterium increased 20.16-fold in response to Alternaria sp., and ITUDI expression increased 3.20-fold in response to Botrytis sp. on day five of incubation. These results were corroborated by gene expression data obtained from B. megaterium during fermentation, where FEND and ITUDI gene expression increased 95.14- and 18.70-fold, respectively. In conclusion, B. megaterium can increase lipopeptide synthesis when exposed to these particular phytopathogens and can significantly increase the respective expression during fermentation.
KW - Antagonism
KW - Antimicrobial genes
KW - Gene expression
KW - Plant pathogens
UR - http://www.scopus.com/inward/record.url?scp=85098957803&partnerID=8YFLogxK
U2 - 10.22207/JPAM.14.4.12
DO - 10.22207/JPAM.14.4.12
M3 - Article
AN - SCOPUS:85098957803
SN - 0973-7510
VL - 14
SP - 2339
EP - 2349
JO - Journal of Pure and Applied Microbiology
JF - Journal of Pure and Applied Microbiology
IS - 4
ER -