PROPAGATION in vitro of QUSIHUAR (Buddleja incana Ruíz & Pav)

Translated title of the contribution: PROPAGATION in vitro of QUSIHUAR (Buddleja incana Ruíz & Pav)

Paola Jiménez Enriquez, Paulo Barrera Aguilar, Laura Huachi Espín, Antonio Vera Zambrano, Carlos Caicedo Vargas

Research output: Contribution to journalArticlepeer-review

Abstract

Quishuar is a forest species which is well known for its uses. Intensive farming and unsatisfied plant supply have been limited to meet reforestation programs. Micropropagation is a technique that would help eradicate this problem as the purpose is to produce more plants in less time. The objective of this research was to develop protocols for disinfection, establishment, and multiplication in vitro; for this reason, tests from seeds and plants sprouts were carried out; the first phase with seeds, using kilol and benomil, together with NaOCl at 3 % (10 min). The results indicate that the germination percentage was 100 % in MS (Murashige y Skoog medium) and the contamination, oxidation percentage was 0 %. On the other hand, plants sprouts exposed to fungicides, antioxidants and NaOCl at 1 % (10 min), did not have contamination or oxidation. The sprouting percentage was 100 % in WPM (Woody Plant Medium). In the second phase in MS medium without the addition of hormones, plant sprouts (1.95 cm), knots number (1.94 knots) and multiplication rate (2.67) were observed. Based on the results, it is suggested that protocols are effective for in vitro propagation.

Translated title of the contributionPROPAGATION in vitro of QUSIHUAR (Buddleja incana Ruíz & Pav)
Original languageSpanish
Pages (from-to)74-84
Number of pages11
JournalGranja
Volume31
Issue number1
DOIs
StatePublished - 1 Jan 2020

Keywords

  • Contamination
  • In vitro
  • Micropropagation
  • Oxidation
  • Quishuar

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