This research was carried out with a sample of leaves and calyxes of Ocotea quixos (Lam.) Kosterm. collected in Archidona, Napo province, Ecuador. Although the bibliography reports the calyxes of the tree as part of the vegetable traditionally used for the extraction of essential oil, there are no data regarding the chemical composition of the essential oil extracted from the leaves. The objective of this work was to extract the essential oil from leaves and calyxes for a comparative study of their physical properties, chemical composition and antimicrobial properties. The residual moisture content in leaves and calyxes was 8 and 12 % respectively. The results of the phytochemical screening carried out on the aqueous and alcoholic extracts of the leaves and calyxes allowed the identification of saponins, reducing sugars, phenols and tannins, and flavonoids. The extraction of the essential oil was done by hydrodistillation, the yield was 1.24% in calyxes after 2 hours of distillation and in the case of leaves of 0.88%. Gas chromatographic analysis of calyx essential oil revealed the presence of two important peaks with retention times of 12,213 and 13,718 minutes; while in leaf essential oil two important peaks were also observed with a slight variation in their retention times: 12,117 and 13,677 minutes, it was assumed that from the qualitative point of view, the two parts of the analyzed plant material present a very similar composition.