Identificación Molecular Del Género Passiflora (P Assifloraceae), En La Región Norte Del Ecuador Mediante El Método DNA Barcoding

Translated title of the contribution: Molecular Identification Of The Passiflora Genus (P Assifloraceae), In The Northern Region Of Ecuador By The DNA Barcoding Method

Research output: Contribution to journalArticle

Abstract

The genus Passiflora or also called passion flower, in Ecuador we have records of 95 species, in this research we collected random samples of species of this group located in the northern region of Ecuador in the provinces of: Pichincha, Santo Domingo, Napo, Tungurahua, Cotopaxi; at altitudinal levels ranging from 400 to 2800 meters above sea level.m, the samples were identified at species level by comparison with vouchers of the National Herbarium of Ecuador QCNE, for the molecular identification the DNA was extracted by means of the method of Doyle and Doyle, then a segment of the matK gene was amplified by means of the conventional PCR technique, the PCR products were sequenced by means of the Sanger technique, the sequences were analyzed with the tools Blast and Boldsystem to identify to which species they correspond, being Blast the best tool for identification. Next, a phylogenetic analysis was made using the matK gene segments and the rcbL segment information available in Genbank. 3 trees were elaborated, one for each segment and one concatendanting the trees constructed by the matk segment and the concatenated show similarity in the grouping of species in agreement with the morphology.
Translated title of the contributionMolecular Identification Of The Passiflora Genus (P Assifloraceae), In The Northern Region Of Ecuador By The DNA Barcoding Method
Original languageSpanish (Ecuador)
JournalBionatura
Volume01
Issue numberBionatura Conference Serie
DOIs
StatePublished - 15 Nov 2018

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