Identificación Molecular del Género Passiflora (PASSIFLORACEAE), en la Región Norte del Ecuador Mediante el Método Dna Barcoding

The genus Passiflora or also called passion flower, in Ecuador has records of 95 species, in this research were collected randomly samples of species of this group located in the northern region of Ecuador in the provinces of: Pichincha, Santo Domingo, Napo, Tungurahua, Cotopaxi; in altitudinal floors ranging from 400 to 2800 m. a.s. l. m, the samples were identified to species level by comparison with vouchers from the National Herbarium of Ecuador QCNE, for molecular identification DNA was extracted by the method of Doyle and Doyle, then a segment of the matK gene was amplified by conventional PCR technique, the PCR products were sequenced by the Sanger technique, the sequences were analyzed with Blast and Boldsystem tools to identify which species they correspond to, Blast being the best tool for identification. Next, a phylogenetic analysis was performed using the segments of the matK gene and the information of the rcbL segment that is available in Genbank, 3 trees were elaborated, one for each segment and one concatenating the trees constructed by the matK segment and the concatenated one show similarity in the grouping of species in concordance with the morphology.