Isolation and Quantification of Three Genes Involved in the Expression of Anthocyanins in Rubus Glaucus Benth by Rt-qpcr

Viviana Pamela Chiluisa Utreras, Ivonne De Los Angeles Vaca Suquillo, Oswaldo Javier Chicaiza Castillo, Sofia Alexandra Peñaherrera Toledo, Ramiro Daniel Acurio Vasconez

Research output: Contribution to journalArticle


The violet and purple colored fruits contain phenolic compounds specifically, anthocyanins. These have a high nutritional quality and anthocyanins are believed to have anti-aging, anti-carcinogenic and antioxidant properties in the fruit. Gene expression studies have demonstrated the importance of flavonoids and their biosynthetic pathways, further increasing the interests of research and sources. In the present study, for the isolation and quantification of the β-Actin, RuANS and RuMYB10 genes involved in the expression of anthocyanins in the cascade of Rubus glaucus Benth, the Real-Time Polymerase Chain Reaction Reverse transcription (RT-qPCR). According to the values obtained with the statistical analyzes DCA and the Turkey test at 95% confidence, observed a relationship between the genes as no significant differences existed in the expression of the three, generating the following results: Actin presented higher concentration (273.42ng/mL), followed by the RuMYB10 gene (220.33ng/mL) and finally the Ruans gene (212.75ng/mL), concluding that the species studied had similar levels of expression Three genes analyzed.
Translated title of the contributionAislamiento y Cuantificación de Tres Genes Involucrados en la Expresión de Antocianinas en Rubus Glaucus Benth por Rt-qpcr
Original languageEnglish (US)
Pages (from-to)314-316
Number of pages3
JournalMoj Food Processing & Technology
Issue number5
StatePublished - 8 Dec 2017


  • Anthocyanins
  • Rt-qpcr
  • Ruans gene
  • Rubus glaucus benth
  • Rumyb10 gene
  • Β-actin gene

CACES Knowledge Areas

  • 215A Biochemistry


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