TY - JOUR
T1 - First molecular identification of hydatid tapeworm Echinococcus granulosus sensu lato G6/G7 in Ecuador
AU - Ramos-Sarmiento, D.
AU - Chiluisa-Utreras, V.
PY - 2019/11/4
Y1 - 2019/11/4
N2 - Echinococcosis is a zoonotic parasitic illness that can cause significant disabilities, and even death for sick people. The disease is caused by the larval stage of cestodes belonging to the Echinococcus genus. In this study, multiple hydatid cysts were excised from an infected porcine liver. The identification of the parasitic species was made by the morphometric assessment of rostellar hooks and molecular detection of ribosomal DNA extant in protoscoleces of the hydatid sand. Rostellar hooks presented an average length of 27.4 µm by optical microscopy. Parasite DNA were detected in samples of hydatid sediment and positive controls by polymerase chain reaction. In conclusion, Echinococcus granulosus was recognized in samples of porcine hydatid cysts by microscopic observation, and the E. granulosus sensu lato strain E. canadensis G6/G7 was identified by molecular assay.
AB - Echinococcosis is a zoonotic parasitic illness that can cause significant disabilities, and even death for sick people. The disease is caused by the larval stage of cestodes belonging to the Echinococcus genus. In this study, multiple hydatid cysts were excised from an infected porcine liver. The identification of the parasitic species was made by the morphometric assessment of rostellar hooks and molecular detection of ribosomal DNA extant in protoscoleces of the hydatid sand. Rostellar hooks presented an average length of 27.4 µm by optical microscopy. Parasite DNA were detected in samples of hydatid sediment and positive controls by polymerase chain reaction. In conclusion, Echinococcus granulosus was recognized in samples of porcine hydatid cysts by microscopic observation, and the E. granulosus sensu lato strain E. canadensis G6/G7 was identified by molecular assay.
KW - DNA sequence
KW - Ecuador
KW - genus Echinococcus
KW - hydatid
KW - rostellar hooks
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85074412807&origin=inward
UR - https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85074412807&origin=inward
UR - http://www.mendeley.com/research/first-molecular-identification-hydatid-tapeworm-echinococcus-granulosus-sensu-lato-g6g7-ecuador
U2 - 10.1017/S0022149X19000944
DO - 10.1017/S0022149X19000944
M3 - Article
SN - 1475-2697
VL - 94
SP - e100
JO - Journal of Helminthology
JF - Journal of Helminthology
ER -