TY - JOUR
T1 - Evaluation of bacillus megaterium strain AB4 as a potential biocontrol agent of alternaria japonica, a mycopathogen of Brassica oleracea var. italica.
AU - Acurio Vásconez, Ramiro Daniel
AU - Tenorio Moya, Estefany Michelle
AU - Collaguazo Yépez, Leidy Andrina
AU - Chiluisa-Utreras, Viviana Pamela
AU - Vaca Suquillo, Ivonne de los Ángeles
N1 - © 2020 The Authors.
PY - 2020/6
Y1 - 2020/6
N2 - Alternaria japonica causes annual losses of up to 25 % of the world broccoli crops, for this reason this research focused on the development of biopreparations containing Bacillus megaterium to prevent the outbreak of this disease caused by Alternaria japonica in the crop of Brassica oleracea var. italica. During the laboratory phase two types of biopreparations were evaluated, the first biopreparation was obtained by liquid fermentation composed of 40 g.L−1 of fava bean flour and 5 g.L−1 of ground brown sugar. This showed a maximum cell growth of 3.8 × 108 CFU. mL−1; while the second biopreparation was obtained by solid fermentation composed of wheat bran and it achieved a maximum cell growth of 4.7 × 109 CFU. g−1. In the fieldwork phase the aforementioned biopreparations were applied in an open-field crop. At the end of the cultivation period, the degree of the disease in leaves and in the inflorescences was measured and through the statistical analysis, a significant difference was evidenced (α = 0.05). On the broccoli leaves the disease index values do not exceed 15.56 % and the disease index for postharvest florets was around 38 %. The evaluated variables showed a statistical similarity with the chemical treatment, thus determining the effective effect of the biopreparations.
AB - Alternaria japonica causes annual losses of up to 25 % of the world broccoli crops, for this reason this research focused on the development of biopreparations containing Bacillus megaterium to prevent the outbreak of this disease caused by Alternaria japonica in the crop of Brassica oleracea var. italica. During the laboratory phase two types of biopreparations were evaluated, the first biopreparation was obtained by liquid fermentation composed of 40 g.L−1 of fava bean flour and 5 g.L−1 of ground brown sugar. This showed a maximum cell growth of 3.8 × 108 CFU. mL−1; while the second biopreparation was obtained by solid fermentation composed of wheat bran and it achieved a maximum cell growth of 4.7 × 109 CFU. g−1. In the fieldwork phase the aforementioned biopreparations were applied in an open-field crop. At the end of the cultivation period, the degree of the disease in leaves and in the inflorescences was measured and through the statistical analysis, a significant difference was evidenced (α = 0.05). On the broccoli leaves the disease index values do not exceed 15.56 % and the disease index for postharvest florets was around 38 %. The evaluated variables showed a statistical similarity with the chemical treatment, thus determining the effective effect of the biopreparations.
KW - Biological control
KW - Biopreparation
KW - Broccoli
KW - Mycopathogen
UR - http://www.scopus.com/inward/record.url?scp=85084141742&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/13ec2413-1aa1-34c3-ad80-13c81496239e/
U2 - 10.1016/j.btre.2020.e00454
DO - 10.1016/j.btre.2020.e00454
M3 - Article
C2 - 32395436
AN - SCOPUS:85084141742
SN - 2215-017X
VL - 26
SP - e00454
JO - Biotechnology Reports
JF - Biotechnology Reports
M1 - e00454
ER -