Estandarización de Protocolos para la Extracción de Adn Cromosómico en Cepas de Colletotrichum Gloeosporioides Aislados en Plantas de Mango (Mangifera Indica L.)

Colletotrichum gloeosporioides (Penz) Penz & Sacc. is the species responsible for anthracnose in mango; this disease affects the entire aerial part of the plant, causing losses of up to 12% of production. Microbial identification and characterization is important for taking measures to prevent and control the pathogen in plantations. The objective of this research was to standardize protocols for the isolation of genomic DNA from strains of Colletotrichum gloeosporioides (Penz) Penz & Sacc., to guarantee quality and concentration for future molecular work on different representatives of this fungal species. The isolation of genomic DNA was performed by three methodologies: CTAB Doyle and Doyle (1990), Kuramane-Izioka (1997) with modifications and CTAB California modified by Ramírez et al., (2004) conjugated with NucleoSpin commercial reagent kits. The quality of the DNA obtained was checked by absorbance measured at 260 and 280nm. The first method was not effective, while the other methods obtained genomic DNA with the quality and concentrations adequate for molecular research with the species. The modifications made to the Kuramane-Izioka method (1997) contributed to its effectiveness. The CTAB California method modified by Ramírez et al. (2004) constitutes the first report of its use for representatives of the Fungi kingdom.