Efecto del carbón activado en la germinación y brotación in vitro de Citrus limon (L.) y su dinámica de crecimiento

Ivonne Vaca; Miguel Marulanda; Joana Verdesoto; Alejandro Núñez; Ramiro Daniel Acurio; Viviana Chiluisa-Utreras

doi:10.21931/RB/2018.03.03.5

Efecto del carbón activado en la germinación y brotación in vitro de Citrus limon (L.) y su dinámica de crecimiento

Translated title of the contribution: Effect of activated charcoal on in vitro germination and shoot multiplication of Citrus limon (L.) and its growth dynamics

Ivonne Vaca
, Miguel Marulanda
, Joana Verdesoto
, Alejandro Núñez
, Ramiro Daniel Acurio
, Viviana Chiluisa-Utreras

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Tissue culture of Citrus limon has focused on the influence of growth regulators for their propagation. In the present study, the evaluation of the culture medium supplemented with activated charcoal presented better results than the addition of plant growth regulators. The in vitro culture of C. limon in Murashige and Skoog (MS) medium added with activated charcoal (1 g.L-1), allowed to get favorable results in days after germination and shoot multiplication. In addition, the curves were generated for germination (introduction phase), stem length, number of shoots per plant, number of leaves per plant and leaf length (multiplication phase), these parameters allows to follow the growth dynamics. Finally, the correlation of the increase in the number of shoots and stem length was determined.

Translated title of the contribution	Effect of activated charcoal on in vitro germination and shoot multiplication of Citrus limon (L.) and its growth dynamics
Original language	Spanish
Pages (from-to)	657-664
Number of pages	8
Journal	Revista Bionatura
Volume	3
Issue number	3
DOIs	https://doi.org/10.21931/RB/2018.03.03.5
State	Published - 2018

Bibliographical note

Publisher Copyright:
© 2018 Centro de Biotecnologia y Biomedicina, Clinical Biotec. Universidad Católica del Oriente (UCO), Univesidad Yachay Tech. All right reserved.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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10.21931/RB/2018.03.03.5

Cite this

@article{88ead554b889485d87bcea79d74c375b,

title = "Efecto del carb{\'o}n activado en la germinaci{\'o}n y brotaci{\'o}n in vitro de Citrus limon (L.) y su din{\'a}mica de crecimiento",

abstract = "Tissue culture of Citrus limon has focused on the influence of growth regulators for their propagation. In the present study, the evaluation of the culture medium supplemented with activated charcoal presented better results than the addition of plant growth regulators. The in vitro culture of C. limon in Murashige and Skoog (MS) medium added with activated charcoal (1 g.L-1), allowed to get favorable results in days after germination and shoot multiplication. In addition, the curves were generated for germination (introduction phase), stem length, number of shoots per plant, number of leaves per plant and leaf length (multiplication phase), these parameters allows to follow the growth dynamics. Finally, the correlation of the increase in the number of shoots and stem length was determined.",

keywords = "Activated charcoal, Germination, Growth curves, Lemon",

author = "Ivonne Vaca and Miguel Marulanda and Joana Verdesoto and Alejandro N{\'u}{\~n}ez and Acurio, \{Ramiro Daniel\} and Viviana Chiluisa-Utreras",

year = "2018",

doi = "10.21931/RB/2018.03.03.5",

language = "Espa{\~n}ol",

volume = "3",

pages = "657--664",

journal = "Revista Bionatura",

issn = "1390-9347",

publisher = "Clinical Biotec, Universidad Catolica del Oriente (UCO)",

number = "3",

}

TY - JOUR

T1 - Efecto del carbón activado en la germinación y brotación in vitro de Citrus limon (L.) y su dinámica de crecimiento

AU - Vaca, Ivonne

AU - Marulanda, Miguel

AU - Verdesoto, Joana

AU - Núñez, Alejandro

AU - Acurio, Ramiro Daniel

AU - Chiluisa-Utreras, Viviana

PY - 2018

Y1 - 2018

N2 - Tissue culture of Citrus limon has focused on the influence of growth regulators for their propagation. In the present study, the evaluation of the culture medium supplemented with activated charcoal presented better results than the addition of plant growth regulators. The in vitro culture of C. limon in Murashige and Skoog (MS) medium added with activated charcoal (1 g.L-1), allowed to get favorable results in days after germination and shoot multiplication. In addition, the curves were generated for germination (introduction phase), stem length, number of shoots per plant, number of leaves per plant and leaf length (multiplication phase), these parameters allows to follow the growth dynamics. Finally, the correlation of the increase in the number of shoots and stem length was determined.

AB - Tissue culture of Citrus limon has focused on the influence of growth regulators for their propagation. In the present study, the evaluation of the culture medium supplemented with activated charcoal presented better results than the addition of plant growth regulators. The in vitro culture of C. limon in Murashige and Skoog (MS) medium added with activated charcoal (1 g.L-1), allowed to get favorable results in days after germination and shoot multiplication. In addition, the curves were generated for germination (introduction phase), stem length, number of shoots per plant, number of leaves per plant and leaf length (multiplication phase), these parameters allows to follow the growth dynamics. Finally, the correlation of the increase in the number of shoots and stem length was determined.

KW - Activated charcoal

KW - Germination

KW - Growth curves

KW - Lemon

UR - https://www.scopus.com/pages/publications/85084110354

U2 - 10.21931/RB/2018.03.03.5

DO - 10.21931/RB/2018.03.03.5

M3 - Artículo

AN - SCOPUS:85084110354

SN - 1390-9347

VL - 3

SP - 657

EP - 664

JO - Revista Bionatura

JF - Revista Bionatura

IS - 3

ER -

Efecto del carbón activado en la germinación y brotación in vitro de Citrus limon (L.) y su dinámica de crecimiento

Abstract

Bibliographical note

UN SDGs

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Evaluation of Photosynthetic Efficiency as an Indicator of Stress in Andean Fruits and Vegetables Cultivated In Vitro (Phase II)

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Efecto del carbón activado en la germinación y brotación in vitro de Citrus limon (L.) y su dinámica de crecimiento

Abstract

Bibliographical note

UN SDGs

Access to Document

Other files and links

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Evaluation of Photosynthetic Efficiency as an Indicator of Stress in Andean Fruits and Vegetables Cultivated In Vitro (Phase II)

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