Efecto del carbón activado en la germinación y brotación in vitro de Citrus limon (L.) y su dinámica de crecimiento

Translated title of the contribution: Effect of activated charcoal on in vitro germination and shoot multiplication of Citrus limon (L.) and its growth dynamics

Ivonne Vaca, Miguel Marulanda, Joana Verdesoto, Alejandro Núñez, Ramiro Daniel Acurio, Viviana Chiluisa-Utreras

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Tissue culture of Citrus limon has focused on the influence of growth regulators for their propagation. In the present study, the evaluation of the culture medium supplemented with activated charcoal presented better results than the addition of plant growth regulators. The in vitro culture of C. limon in Murashige and Skoog (MS) medium added with activated charcoal (1 g.L-1), allowed to get favorable results in days after germination and shoot multiplication. In addition, the curves were generated for germination (introduction phase), stem length, number of shoots per plant, number of leaves per plant and leaf length (multiplication phase), these parameters allows to follow the growth dynamics. Finally, the correlation of the increase in the number of shoots and stem length was determined.

Translated title of the contributionEffect of activated charcoal on in vitro germination and shoot multiplication of Citrus limon (L.) and its growth dynamics
Original languageSpanish
Pages (from-to)657-664
Number of pages8
JournalRevista Bionatura
Volume3
Issue number3
DOIs
StatePublished - 2018

Bibliographical note

Publisher Copyright:
© 2018 Centro de Biotecnologia y Biomedicina, Clinical Biotec. Universidad Católica del Oriente (UCO), Univesidad Yachay Tech. All right reserved.

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