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Comparación de la Variabilidad Genética de Tres Regiones de Adn Cloroplástico y una Nuclear en el Ishpingo (Ocotea Quixos), Proveniente de 5 Provincias de la Amazonía Ecuatoriana

Translated title of the contribution: Comparison of the Genetic Variability of Three Chloroplastic and One Nuclear DNA Regions in the Ishpingo (Ocotea Quixos), Coming from 5 Provinces of the Ecuadorian Amazon
  • Paula Elizabeth Salazar Costa
  • , Patricio Israel Rivas Navarrete
  • , Germania Margarita Karolys Gutierrez

Research output: Contribution to conferencePaper

Abstract

Ocotea quixos (Lam.) Kosterm. (Ishpingo) is a tree that grows in humid tropical forests of South America (310–1200 m above sea level) and is used medicinally and as a condiment, as it possesses essential oils with antiplatelet and antithrombotic properties, among others. In Ecuador, wild populations of Ishpingo are declining due to ecosystem transformation, deforestation, and clonal cultivation. This study compared the genetic variability of three chloroplast DNA regions (matK, rbcLa, and psbA trnH) and one nuclear ITS region from Ishpingo obtained from five provinces of the Ecuadorian Amazon. Based on the alignments obtained, phylogeny was performed using the maximum likelihood criterion, with a bootstrap of 1000 replicates and Jukes-Cantor. Genetic variability was determined using the DNA conservation coefficient and Nei's nucleotide variability coefficient (π). The nucleotide variability of the matK, rbcLa, and psbA-trnH regions was low (π ≤ 0.005) due to their high degree of conservation. The ITS region showed greater variability (π = 0.11), grouping the analyzed individuals into two clades. The results for the ITS region may be affected by the homoplasy present in this region. The analyzed regions were not useful for studying genetic variability in Ishpingo. Therefore, it is suggested that new regions of chloroplast and nuclear DNA be analyzed to allow for population genetic studies of this species in Ecuador.
Translated title of the contributionComparison of the Genetic Variability of Three Chloroplastic and One Nuclear DNA Regions in the Ishpingo (Ocotea Quixos), Coming from 5 Provinces of the Ecuadorian Amazon
Original languageSpanish (Ecuador)
StatePublished - 1 May 2017
EventV Congreso de la Red Ecuatoriana de Universidades y Escuelas Politécnicas para Investigación y Posgrado, REDU 2017 - EC
Duration: 5 Oct 20176 Oct 2017
http://congreso.redu.edu.ec/

Conference

ConferenceV Congreso de la Red Ecuatoriana de Universidades y Escuelas Politécnicas para Investigación y Posgrado, REDU 2017
Period5/10/176/10/17
Internet address

Keywords

  • Genética vegetal
  • Kosterm
  • Ocotea quixos

CACES Knowledge Areas

  • 115A Biology

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