Biotransformation of Hyodeoxycholic and Deoxycholic Bile Acids

María Elena Maldonado Rodríguez, Mishell Enith Araujo Guayasamín, Andrea Alejandra Ruíz Moreno, Stefania Costa

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations


Biotransformations involve the use of microorganisms that allow to modify organic compounds by generating different molecules with possible pharmacological, nutritional, or cosmetic properties. The aim of this research was to evaluate the biotransformations of bile acids (hyodeoxycholic acid, HA, and deoxycholic acid, DA), using microorganisms isolated from slaughtering houses of Ecuador. The biotransformations were analyzed by thin-layer chromatography (TLC) and nuclear magnetic resonance (NMR). The genus identification of the bacteria has been carried out by biochemical tests. The results showed that the more efficient bacteria for the biotransformation of HA were identified as Vibrio alginolyticus, Burk holderia pseudomallei, and Vibrio parahaemolyticus. For DA biotransformations, the most efficient bacteria were Proteus penneri, Burkholdeira pseudomallei, Pasteurella multocida, and Pseudomona aeruginosa. The low biotransformation capacity found in fungi may be due to the difference in metabolic pathways between the two types of microorganisms and that the best source for obtaining bacteria with biotransformation capacity (100%) of HA was the container of intestines, whereas for DA the best sampling area was the slaughtering area, isolating bacteria with a biotransformation capacity of 80%.

Original languageEnglish
Title of host publicationCommunication, Smart Technologies and Innovation for Society - Proceedings of CITIS 2021
EditorsÁlvaro Rocha, Paulo Carlos López-López, Juan Pablo Salgado-Guerrero
PublisherSpringer Science and Business Media Deutschland GmbH
Number of pages10
ISBN (Print)9789811641251
StatePublished - 2022
Event7th International Conference on Science, Technology and Innovation for Society, CITIS 2021 - Virtual, Online
Duration: 26 May 202128 May 2021

Publication series

NameSmart Innovation, Systems and Technologies
ISSN (Print)2190-3018
ISSN (Electronic)2190-3026


Conference7th International Conference on Science, Technology and Innovation for Society, CITIS 2021
CityVirtual, Online

Bibliographical note

Funding Information:
Financing The research has been sponsored by the Salesian Polytechnic University (Ecuador) and the Università di Ferrara (Italy).

Funding Information:
The authors thank the Salesian Polytechnic University (Ecuador) and the Universit? di Ferrara (Italy) for sponsoring the research.

Publisher Copyright:
© 2022, The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd.


  • Bacteria
  • Bile acids
  • Fungi
  • Microorganisms
  • Steroids
  • Thin-layer chromatography


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