Study of the effect on defense genes (PR) by the application of rocoto chili (Capsicum annuum) Solimanillo extract in a tomato (Lycopersicon esculentum L.) crop

This project addresses the need to find alternatives to conventional agrochemicals for pest control in tomato cultivation, seeking solutions that are more environmentally friendly and safer for consumers. The main objective is to investigate whether chili pepper extract (Capsicum annuum) can induce the activation of the plant's natural defense mechanisms, specifically the expression of PR (Pathogenesis-Related) defense genes. The research will be conducted through a controlled greenhouse field experiment using tomato plants (Solanum lycopersicum). A solution of the chili extract will be applied to the plants every seven days. Plant samples will be collected at defined intervals (6, 24, and 48 hours post-application, and subsequently every 30 days) to extract DNA and analyze the genetic expression of PR genes, as well as the concentration of salicylic acid, a key component in plant defense pathways. The resulting data is crucial for validating whether the extract acts as an elicitor, providing a scientific basis for developing more sustainable integrated pest management strategies and reducing crop losses.<br/><br/><b>Goal</b>: <br/>To evaluate the effect of applying chili pepper extract (Capsicum annuum) on the expression of defense-related genes (PR genes) in a greenhouse tomato (Solanum lycopersicum) cultivation.<br/><br/><b>Research lines</b>: <br/>Biodiversity and genetic resources