Potential of Native Bacillus subtilis Strains as a Biocontrol Agent for Alternaria sp in Brassica oleracea var. italica (Phase 3)

This project addresses the challenge of Alternaria spp. diseases in broccoli production, which currently relies heavily on chemical pesticides, leading to resistance and harmful residues. The main goal is to develop a sustainable biological alternative by evaluating native strains of Bacillus subtilis, a bacterium known to produce antifungal compounds like subtilin and iturins. The initiative is in an advanced stage, focusing on formulating compounds based on strains previously validated at laboratory and greenhouse levels. The methodology involves designing substrates for mass propagation of B. subtilis, cell counting, establishing field broccoli crops, applying the biological control agent, and finally evaluating the incidence and severity of Alternaria spp. under the applied treatments. Successful completion will directly benefit producers by providing an effective and ecological tool for integrated pest management.<br/><br/><b>Goal</b>: <br/>To determine the biocontrol efficacy of native Bacillus subtilis strains against diseases caused by Alternaria spp. in broccoli crops.<br/><br/><b>Research lines</b>: <br/>Biodiversity and genetic resources