Molecular Taxonomic Revision of the Genera Caucaea and Restrepia (Orchidaceae)

This project focuses on the exhaustive investigation of the orchid genera Caucaea and Restrepia, genera with high endemic representation in Ecuador, a country renowned for its vast floral biodiversity. The study addresses the molecular and evolutionary characteristics of these species, tackling the need to understand their phylogeny within the context of the Andean region's Orchidaceae family richness. The methodology involves collecting botanical samples across various Ecuadorian provinces and specialized collections. Molecular processing includes DNA extraction and amplification of key segments (matK, rpoC, ITS) using PCR, followed by Sanger sequencing. Bioinformatic analysis is conducted using specialized software like Mega 7, comparing results against public databases such as GEN BANK and BOLD SYSTEMS. Concurrently, biotechnological conservation protocols are implemented, including in vitro culture (from seed acquisition, disinfection, incubation, and hardening) and assisted cultivation in greenhouses. The ultimate purpose is to generate genetic knowledge and develop effective propagation methods for the conservation of these epiphytic orchids.<br/><br/><b>Goal</b>: <br/>The main objective is to study the molecular and evolutionary characteristics of the orchid species belonging to the genera Caucaea and Restrepia. Additionally, the project aims to develop and apply greenhouse and laboratory cultivation techniques to support the conservation efforts for these species.<br/><br/><b>Research lines</b>: <br/>Biodiversity and genetic resources