Molecular Identification of the Etiological Agent of Bovine Mastitis from Samples from Production Units in the Canton of Cayambe, Province of Pichincha

This project addresses bovine mastitis, a multifactorial health issue significantly impacting small and medium-sized dairy producers in the Cayambe canton, Ecuador. Treatment and eradication are complicated by the constant mutation of etiological agents and antibiotic resistance. The main objective is to accurately identify the causative microorganisms by implementing molecular techniques, overcoming the limitations of traditional biochemical methods. The methodology involves DNA extraction using the commercial FastDNA kit from MP Biomedicals. Subsequently, key sequences will be amplified via Polymerase Chain Reaction (PCR) targeting the 16S, rpoB genes, and the ITS region. These molecular markers allow for sensitive and rapid genotypic characterization of the pathogens. The PCR products will be sent for sequencing, and the results analyzed by comparison with databases such as NCBI and BOLD Systems to determine molecular diversity and responsible strains. This molecular approach will provide crucial information for sanitary management and disease control in the region.<br/><br/><b>Goal</b>: <br/>To identify the microorganisms causing bovine mastitis in samples originating from production units in the Cayambe canton, utilizing advanced molecular techniques.<br/><br/><b>Research lines</b>: <br/>animal biotechnology