Molecular Identification of Microorganisms of Biotechnological Interest Using Real-Time PCR Collected in the Natural Regions of Ecuador (Phase 2)

This project focuses on the molecular identification of microorganisms with biotechnological potential, sourced from soil samples across Ecuador's natural regions (Coast, Highlands, Oriente, and Galapagos Islands), originating from a project isolating antibiotic-producing strains. The primary goal is to leverage molecular techniques to identify novel strains, overcoming the limitations of traditional cultivation and avoiding population biases. The methodology involves DNA extraction and purification from pre-incubated samples, utilizing a certified ROCHE kit to ensure compatibility with Real-Time PCR. The purified DNA is subjected to a Real-Time PCR reaction, mixed with a standardized PCR mix including hybridization probes and other essential reagents. Amplification is performed on a LightCycler Instrument over 40 cycles, comparing results against positive controls (ATCC standards) and negative controls (sterile water). This molecular approach enables precise and efficient characterization of microbial diversity relevant for producing economically important metabolites such as antibiotics, pigments, and hormones.<br/><br/><b>Goal</b>: <br/>To molecularly identify microorganisms of biotechnological interest collected from the natural regions of Ecuador using Real-Time PCR techniques.<br/><br/><b>Research lines</b>: <br/>Biodiversity and genetic resources