Influence of Temperature and Carbon Source Concentration on the Bioremediating Capacity of Pseudomonas spp. Against Cadmium and Lead

This research project focuses on deepening the study of microorganisms previously isolated from mining sediments, specifically the bacterium *Pseudomonas luteola*, to evaluate its potential in heavy metal bioremediation. The main objective is to determine the physicochemical conditions (temperature, pH, and carbon source) that maximize this bacterium's capacity to sequester cadmium (Cd) and lead (Pb) present in water. The methodology ranges from sampling industrial effluents and isolating and biochemically characterizing *Pseudomonas spp.* using API 20 NE tests, to molecular identification using PCR and sequencing, comparing results with BLAST. Batch culture assays will be conducted at a 250 mL scale, where growth curves and heavy metal removal rates will be determined using atomic absorption spectrophotometry. Finally, kinetic and removal data will be analyzed using LogNormal regression models and Bayesian paradigms (BIC and BF) to establish the ideal operating conditions for phytoremediation.<br/><br/><b>Goal</b>: <br/>To identify the optimal conditions of temperature, pH, and carbon source concentration at which the bacterium *Pseudomonas luteola* exhibits the highest capacity for removing cadmium and lead from aqueous solutions at a laboratory scale.<br/><br/><b>Research lines</b>: <br/>Evaluation and mitigation of environmental impact