Analysis of Gene Expression of Interest in Biotic Factors with Biotechnological and Agricultural Importance via RT-qPCR (Phase 2)

This project addresses the limitations in biotechnological and agricultural applications, such as the production of pharmaceutical compounds or biocontrol agents, which are hindered by the inefficacy and low yield of living organisms due to suboptimal conditions for gene expression. To resolve this, gene expression quantification via RT-qPCR is implemented, a key molecular technique used to identify ideal conditions that maximize the expression of relevant genes. The methodology involves the selection and preparation of biotic samples, RNA extraction, reverse transcription to obtain cDNA, and precise cDNA quantification using a Qubit 2.0 fluorometer to establish a standard curve. Subsequently, RT-qPCR amplification is performed on the Light Cycler 2.0 equipment, configuring parameters based on the organism and target gene sequences previously selected from bibliographic references. The final analysis allows for the quantification of gene expression, aiding in the identification of potential species that produce metabolites of interest and optimizing biotechnological and agricultural processes.<br/><br/><b>Goal</b>: <br/>To analyze the expression of genes of interest in biotic factors with biotechnological and agricultural importance, utilizing the RT-qPCR technique to identify optimal conditions that enhance gene expression and product yield.<br/><br/><b>Research lines</b>: <br/>Biodiversity and genetic resources